慢性心肌缺血模 z-bo 2021-01-04 346

　　Objective To establish a mouse model of chronic myocardial ischemia by subcutaneously injecting different doses of isoproterenol (ISO) into the neck, and to determine the best model dosage through comparison, to seek more objective and reliable indicators, and to provide references for related model preparation and drug efficacy evaluation.

　　Methods Three model groups were injected subcutaneously with ISO 32mg/kg, 16m/kg and 8mg/kg body mass for 3 consecutive days. Electrocardiograms (ECG) were recorded 24h, 8d and 15d after the model was created; the 8th and 15th days were tested for each Group blood myocardial injury markers and heart samples were taken to observe the pathological changes of myocardial morphology in each group.

　　Results The T wave of the ECG in the ISO32m/kg and ISO16m/kg dose groups was significantly reduced on the 8d and 15d after the model was established (P<0.05 or P<0.01); the myocardial injury markers were significantly changed (P<0.05 or P<0.01) ), in which the ISO32 m/kg group has pathological changes and the mortality rate is 42.5%, which is more suitable for the evaluation of the efficacy of anti-chronic myocardial ischemia drugs for long-term treatment. The mortality rate of ISO16 mg/kg is 25%, which is relatively low and did not cause pathological damage to the myocardium.

　　Conclusion According to different experimental expectations, ISO 16 or 32mg/kg model dose can be selectively used to establish a mouse chronic myocardial ischemia model.