门脉高压动物模型 z-bo 2021-11-11 348

　　Objective To explore whether simple ligation of the left portal vein can form stable portal hypertension and whether nitric oxide plays a corresponding role in it.

　　Methods 36 SPF male 8-week-old SD rats were randomly divided into a left branch ligation group (with a diameter of 0.6, 0.7, and 0.8 mm needles, respectively), and a main ligation group (with a diameter of 0.9 mm needles). Ligation), left lateral lobectomy group, sham operation group, a total of 6 groups. The portal pressure of all rats was measured before operation, immediately after operation, 15 days after operation, and 30 days after operation. All rats were sacrificed 30 days after the operation. The portal vein blood and left and right liver tissues were collected to measure the content of nitric oxide (NO).

　　Results The portal vein pressure of SD rats in the left branch ligation group immediately, 15 days, and 30 days after operation was significantly higher than that before operation and the control group; 30 days after the operation, there was no difference between the left branch 0.6 mm group and the main 0.9 mm group. It is significant; the NO content in the left and right lobes of the liver and blood of the six groups of SD rats were compared and no statistical difference was found.

　　Conclusion Simple ligation of the left portal artery can form stable portal hypertension. A needle with a diameter of 0.6 mm can achieve better results; NO does not play a role in prehepatic portal hypertension.