咳嗽变异型哮喘豚鼠模型 z-bo 2021-01-05 398

　　Objective To try to use ovalbumin combined with smoking to establish a more similar CVA model of guinea pigs.

　　Method A total of 24 SPF male and female guinea pigs were selected, and they were randomly divided into a blank control group, an OVA sensitization group, and an OVA sensitization + smoking group, a total of 3 groups, each with 8 guinea pigs. The OVA sensitization group was modeled by the ovalbumin sensitization method, and the OVA sensitization + smoking group was modeled by the ovalbumin sensitization combined with the smoking method. The rest methods were the same. The lung compliance (Cydn), airway resistance (RI) after methacholine challenge, the number of coughs and the time of first cough after capsaicin challenge, lung tissue pathology and Ashcroft grading index changes in the 3 groups were tested for evaluation. model.

　　Results Compared with the blank control group, the Cydn of the OVA sensitization group and the OVA sensitization + smoking group significantly decreased, the RI increased significantly, the number of coughs at 10 minutes increased significantly, the first cough time was significantly earlier, the lung tissue pathological changes were obvious, and the Ashcroft grading table The score has risen significantly, and the above performances are consistent with the characteristics of cough variant asthma. At the same time, compared to the OVA sensitization group, the OVA sensitization + smoking group has better performance in cough symptoms and changes in RI, and is more in line with the characteristics of cough variant asthma. This may be related to reducing the OVA sensitization concentration to avoid overreaction, Smoke increases cough sensitivity is closely related.

　　Conclusion Using OVA sensitization combined with smoking can establish a cough variant asthma guinea pig model with high similarity.