大鼠颈静脉自主给药插管 z-bo 2021-01-06 305

　　Objective To introduce an improved method of making jugular vein cannula and surgical method of jugular vein cannulation in rats, and apply it in an experimental study of autonomous drug administration in rats to evaluate the service life and application effect of the cannula design.

　　Method 29 male SD rats who have completed food training were used for jugular vein intubation with modified cannula, and a self-administered model of propofol and cocaine was established, and the animals were eliminated 5 months after the operation.

　　Results Both the propofol spontaneous administration model and the cocaine spontaneous administration model were successfully established. The main reasons for the elimination of postoperative animals were dying, tube blockage, and fluid leakage. In the first to 5 months after surgery, they were directly intubated every month. The relevant elimination rates are 7%, 7%, 10%, 31% and 11% respectively. If the monthly elimination rate is within 10%, the service life of the intubation assembly is expected to be at least 3 months. The service life of the intubation can be as long as 5 months or even more than 5 months. The causes of animal dying are related to severe liver and kidney damage, electrolyte imbalance and internal inflammation.

　　Conclusion The improved cannula for autonomous drug administration in the rat jugular vein has many advantages. The intubated rat with the cannula assembly designed in this paper can last as long as 3 months, which can meet the basic needs of autonomous drug administration experiments. Therefore, the application effect is good and can be promoted and used.