动物造模,大鼠动脉粥样硬化痰瘀互结病证结合模型 z-bo 2021-11-09 332

　　Objective: To establish a model of atherosclerosis combining hemorrhoids and congestion, and observe the changes of inflammatory factors and prescription intervention effects of Danlou tablets in model rats.

　　Method: 32 male SD rats were randomly divided into normal group, model group, atorvastatin calcium group (ATV group) and Dunlou group (DLP group), each with 8 rats. The rats in the normal group received basic diet feeding, and the other 3 groups were treated with a combination of high-fat diet + intraperitoneal injection of vitamin D3 and carotid artery balloon injury to reproduce the hemorrhoid and congestive AS model. The model group, ATV group and DLP group were given regular saline, atorvastatin calcium suspension and Denglou tablet suspension for 4 weeks. After intervention, the pathological changes of the rat's thoracic aorta were observed by HE staining, the blood lipids, hs-CRP, IL-6, TNF-α and LP-PLA2 levels in the rat serum were detected, and the rat's thorax was measured by RT-PCR Aorta. The expression of IL-6, TNF-α and LP-PLA2 mRNA in tissues.

　　Results: (1) Serum levels of TC, LDL-C, hs-CRP, IL-6, TNF-α and LP-PLA2 in the model group were higher than those in the normal group (P0.05). When observed under a HE staining optical microscope, the intimal hyperplasia of the thoracic aorta in the ATV and DLP groups was significantly reduced, the area of plaque was reduced, and the degree of atherosclerosis was reduced.

　　Conclusion: The use of high-fat diet + intraperitoneal injection of vitamin D3 combined with carotid artery balloon injury can establish an AS rat model with hemorrhoids and congestion syndrome. Bloodletting and congestion (Danru tablets) are prescribed for this model rat to pass AS and inflammation Improved.