动物造模,大鼠高血流性肺动脉高压模型 z-bo 2021-11-01 363

　　Objective: To establish a rat model of hyperperfusion pulmonary hypertension with common carotid artery and external carotid anastomosis.

　　 Method: Forty-five healthy male SD rats were divided into shunt group, ligation group and sham operation group (n = 15). After 12 weeks, the overall condition of the rat was confirmed, the heart condition, cardiac output and smooth blood flow were observed by echocardiography, the right ventricular systolic pressure was confirmed through the right heart catheter, and blood gas was analyzed. Calculate the pulmonary circulation/systemic circulation blood flow ratio (Qp/Qs). Right ventricular hypertrophy index; HE staining of lung tissue and EVG staining of elastic fibers. Statistical data uses SPSS 16.0 software, and a one-way distributed analysis is performed on each set of data.

　　Result: There was no significant difference in weight gain between the two groups, and the unobstructed anastomosis rate was 84.6%. Compared with the sham operation group and the ligation group, the heart of the rats in the shunt group was significantly enlarged, and the cardiac output was significantly increased ((309.8±33.1) mL/min·Kg to (245.6±31.9) mL/min. ?kg( (240.8±30.9))) mL/min? kg, P\u003c0.05]; the Qp/Qs of the shunt group was 2.16±0.38, and the right ventricular systolic blood pressure increased to (35.8±4.9) mmHg. The right ventricular hypertrophy index in the shunt group was 0.3263±0.0342, which was significantly higher than the other two groups. Compared with the sham operation group, the pulmonary artery media thickness in the shunt group was significantly increased [(22.3±1.7)% to (10.6±1.7)%, P\u003c0.05].

　　 Conclusion: Anastomosis of the common carotid artery and external carotid artery in rats can establish a good model of high pulmonary blood flow and pulmonary hypertension.