动物造模,小鼠脓毒症模型 z-bo 2021-01-08 353

　　Objective: To establish and evaluate the murine sepsis model, to study the pathogenic mechanism of sepsis, and to provide model animals for the development of anti-inflammatory drugs.

　　 Method: Use the CLP method, animal survival rate, postoperative bacterial load, blood routine and blood biochemical indicators, cytokine levels, and histopathological methods to induce septicemia in mice to treat changes. Evaluation model.

　　Result: The mortality of mice is closely related to the cecal ligation site. The survival rate of 50% blindly ligated mice was about 40% at 12 days, while 75% of mice died at 4 days (P\u003c0.01). Compared with the sham operation group, 50% ligated CLP mice increased blood and abdominal cavity bacteria levels and decreased white blood cells. The difference is significant (P\u003c0.001). The levels of liver transaminase ALT, AST and serum urea nitrogen BUN in CLP mice increased, and the difference was statistically significant (P\u003c0.01). The levels of inflammatory factors IL1α, IL6, IL10, MIP1α, MIP1β, and TNFα increased. 48 hours after the operation, the liver and lungs of the mice showed obvious histopathological damage.

　　 Conclusion: The mouse CLP model has typical pathological features of sepsis, which provides a suitable animal model for future anti-inflammatory drug research.