可诱导性肝损伤模型,动物造模 z-bo 2021-01-08 363

　　Objective: To establish an Alb-cre/DTR double transgenic mouse model and analyze the related phenotypes. On this basis, we will establish an inducible liver injury model for related research on liver disease.

　　Method: After breeding the introduced Alb-cre mice and DTR mice, double transgenic mice are obtained by mating. Extract DNA from mouse tail tissues and use PCR to identify genotypes. Different toxins were injected into the abdominal cavity of double-transgenic mice, and then weighed and blood samples were collected at different time points to detect serum ALT and AST levels.

　　Result: Alb-cre and DTR mice were bred and screened to obtain Alb-cre/DTR double transgenic mice. Using 0.625g/g diphtheria toxin in mice can produce ALT in mouse serum. The level of AST increased significantly, and after dissection of the mice, the liver was observed to turn white. The results of HE staining showed obvious necrosis of liver cells.

　　 Conclusion: We have successfully established a mouse model that can induce specific liver damage.