动物造模,小鼠异位肢体复合组织移植模型 z-bo 2021-01-08 285

　　Purpose: To establish a stable heterotopic limb complex tissue transplantation model in mice.

　　Method: 25 pairs of C57BL/6 mice were selected for the transplantation of heterotopic limb complexes of the same blood. The hind limbs of the donor mice were collected and released from the lower end of the inguinal ligament along the femoral artery and femoral vein. All branch vessels except for the femoral vein and femoral artery were electrocoagulated into external arteries and external veins. Cut it open and cut off the external artery and bone. After the external vein, it passes through the femur and femur muscle. The external artery and external vein of the donor were anastomosed end-to-end with the right common carotid artery and external jugular vein of the receiving mouse, respectively. Continuously suture the skin edge of the donor to the skin edge of the recipient to fix the limb. Cut the distal tibial joint of the donor limb and ligate the stump.

　　 Results: The surgical success rate was 76%, the total operation time was (87.1±10.1) minutes, the donor acquisition time was (23.8±4.9) minutes, and the vascular anastomosis time was (55.3±9.6) minutes.

　　 Conclusion: The transplantation of complex tissues of heterotopic limbs in mice has a high success rate and is stable and reliable.