动物造模,缺氧缺血脑损伤大鼠模型 z-bo 2021-01-08 550

　　OBJECTIVE: To simulate the process of neonatal hypoxic-ischemic brain injury in a high-altitude low-pressure environment indoor simulation room, and establish rat models of hypoxic-ischemic brain injury in rats at different altitudes.

　　 Method: 32 10-day-old SD neonatal rats were randomly divided into 4 groups, namely group A (control group) and 3 experimental groups: group B (group 2000m), group C (group 4000m), and group D were divided into groups. (6000m group). The rats in the control group were bred in a barrier environment, and the rats in the experimental group were placed in a high-altitude and low-pressure environment to simulate a cabin and exercise combined to establish a neonatal high-altitude cerebral hypoxia-ischemia model. The exercise method is to swim in the cabin tank for 60 minutes a day. Exercising and living in a simulated warehouse in a plateau low pressure environment for more than 20 hours a day. The ZeaLonga 5-point system was used to score the behavior of each group of rats on days 3, 7, 11, and 15, venous blood was collected on the 15th day, and red blood cell morphology was scanned. Observe with an electron microscope. After sacrificing each group, the brain tissue was harvested for HE and TTC staining.

　　 Results: (1) Neurological score: The behavior scores of experimental groups B, C and D are significantly different from those of the control group (P\003c0.05), and group D is compared with the control group. .. The difference is very important (P\u003c0.01). (2) HE staining results showed that the experimental group had inflammatory cell infiltration compared with the control group, and the degree of inflammatory cell infiltration was positively correlated with the control group. (3) TTC staining shows that cerebral cortex ischemia is obvious in high altitude rats. (4) When observing the morphology of red blood cells with an electron microscope, the experimental group B has a cap shape, the C group has an irregular shape, and the D group has a zigzag shape.

　　 Conclusion: In this study, a high-altitude low-pressure environment simulation chamber combined with exercise to simulate a high-altitude environment created a neonatal hypoxic-ischemic encephalopathy (HIE) model. The model is stable, reliable, and more consistent with ischemic hypoxic brain injury. Near-clinical causes can be used in related research.