动物造模,周期性麻痹模型 z-bo 2021-01-08 377

　　OBJECTIVE: To use gene knock-in CaV1.1-R528H mice to establish and evaluate the hypokalemic hypokalemia periodic paralysis model.

　　Methods: 8-week-old gene knock-in CaV1.1-R528H male mice and 36 8-week-old wild C57BL/6J male mice were used according to body weight with 3 factors and 2 levels 2×2×2 factor design method according to the random principle. Divided into eight categories (the three factors are mutations, tyrosine and insulin, with and without two levels). Among them, mice in the tyrosine treatment group were intraperitoneally injected with levothyroxine sodium weighing 350 μg/kg for 12 consecutive days to treat hyperthyroidism. After the last administration, 0.8 U of short-acting insulin was given to the insulin treatment group. 1kg body weight was injected intraperitoneally. The serum potassium of each group of mice was detected and recorded before injection (0 minutes) and after injection (30, 60 minutes).

　　Results: (1) Mice with hyperthyroidism showed irritation, irritation and drying clothes, and compared with the control group, food and water consumption increased significantly, and weight increased. It's getting late. In thyroid function tests, T3 and T4 were significantly higher than the corresponding control group, and TSH was significantly lower than the corresponding control group, the difference was statistically significant (P\u003c0.05). (2) When tyrosine or insulin was used alone, there was no significant difference in serum potassium between the mutant group and the wild group at the same time point, but after high tyrosine insulin treatment, the mutant group and the wild group were at the same time point. (30, 60 minutes) The serum potassium of the mutant group was significantly lower than that of the wild type group (P\u003c0.05). (3) Main effects and interactions: Single mutation factor or tyrosine factor does not affect serum potassium, only insulin affects the reduction of serum potassium (P\u003c0.05); between tyrosine factor and sudden mutagen and There is an interaction between insulin factor and mutagen (P\u003c0.05); there is no interaction between tyrosine factor and insulin factor.

　　Conclusion: (1) The treatment of hyperthyroidism was successful. (2) Gene knock-in CaV1.1-R528H mice successfully established a hypokalemic hypokalemic periodic paralysis model.