动物造模,2型糖尿病小鼠模型 z-bo 2021-01-08 398

　　Objective: To compare the differences in glucose metabolism, insulin resistance and inflammation between spontaneous and induced type 2 diabetes mouse models.

　　Method: The animals are divided into a normal control group, an induction model group and a spontaneous model group, with 10 animals in each group. Normal control group: normal 11-week-old C57BL/6J mice; induction model group: use high-fat diet combined with STZ to induce 11-week-old C57BL/6J mice; establish spontaneous diabetes model group: 8 select one-week-old C57BL/ KsJ-db/db mice; monitor the weight, fasting blood glucose and glucose tolerance changes of each group before and after the experiment, as well as FINS, AUC, HOMA-IR, TNF-α, IL detection-18, IL-1β and Changes in INF-γ content. After 8 weeks, the animals were sacrificed to collect liver, kidney, pancreas, testis, skeletal muscle and other tissues for pathological analysis.

　　Result: During the experiment, the body weight of the spontaneous model group continued to increase, but the weight of the induced model group was significantly reduced; the blood glucose of the induced model group decreased after 8 weeks, but the spontaneous model group did not. Stable maintenance of hyperglycemia; model FINS induced by spontaneous model group increased, AUC and HOMA-IR decreased significantly. The FINS of the spontaneous model group did not change significantly. Both AUC and HOMA-IR increased significantly. At the beginning of the model establishment, the levels of serum inflammation-inducing factors (TNF-α, IL-18, IL-1β, INF-γ) were higher than those of the normal control group. In the 8th week, the stimulating factor content of the stimulating model group decreased, and the proliferation factor content of the stimulating model group increased significantly. The pancreas and other tissues of the two model mice had obvious inflammatory lesions.

　　 Conclusion: After 8 weeks, the abnormal glucose metabolism and inflammation of the spontaneous type 2 diabetes model gradually increase, and the symptoms of hyperglycemia can be maintained for a long time. After 8 weeks of feeding, glucose metabolism and inflammation in the induced type 2 diabetes model were partially alleviated, and hyperglycemia symptoms were partially improved.