酒精性肝硬化,动物造模 z-bo 2022-02-02 434

　　[Modeling mechanism] Acetaldehyde is an intermediate metabolite of ethanol, which directly damages the liver and converts coenzyme I (NAD) into reduced coenzyme I (NADH) in the liver, thereby reducing the ratio of NAD/NADH. It inhibits the tricarboxylic acid cycle, and the oxidation of fat is weakened, resulting in fatty liver, and eventually liver fibrosis and cirrhosis.

　　[Modeling method] Simultaneously give a mixture of ethanol (56%) and olive oil to rats. The initial dose of white wine to rats is 8g/kg, increasing by 2g every 2 weeks to 16g/kg.

　　[Model Features] Under an electron microscope, the central area of the liver lobules was obviously necrotic within 4 weeks of modeling, with local or punctate necrosis, inflammatory infiltration in the interstitium, and liver cell circumference. Proliferation of collagen fibers was observed.

　　[Evaluation and application of the model] The incidence of liver fibrosis is high, the model is simple, and the model is stable, which can be widely used in the study of alcoholic liver disease. Can be used as liver fiber.