动物造模,小鼠哮喘模型 z-bo 2021-01-08 521

　　Objective: To observe the effect of pe glucoside on airway inflammation chemokines and their receptors in a mouse asthma model.

　　 Method: Use ovalbumin (OVA) to sensitize and challenge the establishment of a mouse asthma model. Serum IL-6, TNF-α levels and bronchoalveolar lavage fluid (bronchoalveolar lavage fluid, BALF) ELISA method to detect egg protein specific IgE (OVA)-IgE) and chemokine CCL19, CCL21 levels; T -PCR method to detect human CCR7 mRNA expression; Western blot method to detect lung tissue CCR7 and nuclear factor-κB (nuclearfactor-κB, NF-κB) protein expression.

　　Results: The serum IL-6 and TNF-α levels of mice in the Pe drug intervention group were significantly reduced; BALF OVA-IgE, CCL19 and CCL21 levels were significantly reduced; the lung tissue CCR7 mRNA, CCR7 and NF-κB expressions were significantly reduced.

　　 Conclusion: Pe drug glycosides have a significant inhibitory effect on the airway inflammatory chemokine CCL19/CCL21 and its receptor CCR7 in asthma model mice.