动物造模,胚绒膜尿囊膜移植瘤模型 z-bo 2021-11-26 464

　　OBJECTIVE: To compare the growth of three different cancer cell lines in chicken chorionic villus (CAM), screen the best transplanted cancer cell lines, establish transplanted tumor models and observe their biological characteristics. Methods: 7-day-old chicken embryo CAM were respectively inoculated with human lung cancer A549 strain, tongue squamous cell carcinoma TCA8113 strain and human liver cancer QGY7703 strain, and the survival rate of each chicken embryo, tumor strain survival rate, tumor formation rate, Induce angiogenesis to test other indicators, observe and screen the growth characteristics of transplanted tumor models.

　　 Results: Compared with human lung cancer A549 strain and liver cancer QGY7703 strain, tongue squamous cell carcinoma TCA8113 strain has the highest tumor formation rate (P\u003c0.05), and is the best cancer cell line for transplantation. The optimal number of cancer cells to be inoculated is 8.0. ×106/tablet, the best tumor growth cycle after inoculation is 4-8 days, and the best experimental time is 7 days after inoculation.

　　 Conclusion: TCA-CAM transplanted tongue squamous cell carcinoma tumor model has been established and established. It provides an excellent experimental animal model for the detailed study of the biological characteristics of tumor growth, angiogenesis, invasion and metastasis mechanisms, and the screening of anti-tumor drugs.