动物造模,EV71病毒对树鼩原代肾上皮细胞感染模型 z-bo 2022-01-22 466

　　Objective: To establish an EV71 infection model of primary kidney cells in tree breaks.

　　Methods: The primary kidney cells of tree sh were obtained by trypsin digestion, the kidney cells of tree cells were infected with EV71, and Western blot and indirect immunofluorescence methods 1, 2, 4 were used to measure the culture on days 6, 8 and 8. Virus titer of the serum. The expression of EV71 virus VP1 protein in cells was detected to determine the infectivity of EV71 virus to shrimp primary kidney cells.

　　Result: The primary kidney cells isolated by Tree Schrew were passaged, purified, and morphologically identified, and a cell culture based on Tree Schrew's primary kidney cells was established. When EV71 virus infects primary kidney cells infected by trees, the virus titer can reach 1.3×106 TCID50/mL 48-96 hours after infection, and EV71 virus can reach primary kidney cells infected by trees. It shows that it can be effectively infected and spread effectively. By Western blot detection, the EV71 virus VP1 protein can be effectively detected in tree primary kidney cells 2 to 8 days after infection, and the distribution of virus VP1 protein in the cytoplasm 2 to 6 days after infection can be detected by indirect immunofluorescence. Has been completed.

　　Conclusion: Based on the successful establishment of the primary kidney cell culture of chopped trees, the infectivity and virus growth characteristics of EV71 virus in primary kidney cells of chopped trees were determined, and the primary kidney of EV71 chopped trees was determined. The cell infection model has been established for the first time.