动物造模,除术后急性肝功能衰竭模型 z-bo 2021-01-09 353

　　Objective: To investigate the effect of different proportions of hepatectomy volume on acute liver failure in Bama mini-pigs, and to establish an appropriate model for most models of acute liver failure after hepatectomy in mini-pigs.

　　 Method: Excise 75%, 85% and 95% of the liver, and check the remaining liver by CT and record the survival status. Blood was collected regularly before the operation, 1, 3, 5 days, 1, 2 and 3 weeks after the operation. It works by obtaining HE staining of liver tissue and checking liver pathology.

　　Result: The average survival time of minipigs undergoing liver resection was (19.0±5.6) days, (17.3±5.5) days and (1.3±1.5) days, respectively, 75%, 85% and 95%. The pathological scores of Bamani pigs with different liver resection rates were (5.67±0.52), (8.17±0.82) and (8.50±0.71). The higher the liver resection rate, the higher the incidence of liver failure. Eighty-five percent of liver resection can significantly increase the levels of alanine aminotransferase, aspartate aminotransferase, alkaline phosphatase, lactate dehydrogenase and total bile acids.

　　 Conclusion: 85% volume resection can lead to a typical acute liver failure model of miniature pigs.