动物造模,猪动脉粥样硬化模型 z-bo 2021-01-09 375

　　Objective: To establish a high-fat-induced Wuzhishan mini-pig atherosclerosis model and observe the expression changes of lipoprotein-related phospholipase A2 (Lp-PLA2) in plasma and plaques.

　　Method: Wuzhishan miniature pigs were randomly divided into normal control group (Ctr), 4 were fed normal diet and ASmodel group were fed high-fat diet for 24 weeks. Twenty-four weeks after modeling, blood was taken from the fasting anterior aorta to collect total cholesterol (TC), low density lipoprotein (LDL-C), high density lipoprotein (HDL-C), and triglycerides (TG). ) And measured C-reactive protein. (CRP), changes in the activity and composition of Lp-PLA2, oil red O stained aorta, HE staining and IL-6 immunohistochemical staining of abdominal aortic vessels, vascular lipid deposition and plaque observation pathology. Changes in IL-6 protein expression. RT-PCR and Western Blot were used to observe the expression of Lp-PLA2 mRNA and protein in the abdominal aorta.

　　 Results: Compared with the normal control group, the weight, body mass index (BMI), TC, LDL-C, HDL-C, CRP, Lp-PLA2 activity and composition of the AS model group increased significantly. (P\u003c0.05, P\u003c0.01) At the same time, the aortic lipid deposition was obvious (P\u003c0.01) and AS plaques were formed. The expression of Lp-PLA2 mRNA, protein and IL-6 protein in abdominal aorta tissue was significantly increased (P\u003c0.05).

　　 Conclusion: A 24-week long-term high-fat diet can induce AS in Wuzhishan miniature pigs. Lp-PLA2 plays an important role in vascular inflammation and AS plaque formation.