动物造模 z-bo 2022-02-02 355

　　[Modeling mechanism] By blocking the bile duct or injecting sclerosing agent, the extrahepatic bile duct is artificially blocked, causing the bile duct to expand above the blocked site, cholestasis, and increasing the pressure of the bile duct. I will. Intrahepatic blood vessels are dilated by bile ducts and bile overflows, bile pigmentation, degeneration and necrosis in liver cells are oppressed, fibrous tissue spreads to bile ducts, lobules are reconstructed, liver fibrosis and cirrhosis.

　　[Modeling method] The bile duct is ligated with silk thread through the abdomen. Circulate the common bile duct at two points 1 cm apart using 6 to 0 silk threads. Four days after the operation, normal feeding will be performed. The amount of food is 20-21g/d, and the model is prepared after 6 weeks of feeding. This allows you to create experimental models of biliary fibrosis and cirrhosis in dogs, rats, monkeys and other animals.

　　[Model Features] Inflammation is mild, hepatocyte necrosis in liver tissue is not obvious, except for the mild infiltration of monocytes into the portal area, there are few inflammatory cells. Liver fibrosis develops rapidly and occurs about 4 weeks after bile duct obstruction. 16 weeks after modeling, the spontaneous recovery rate was low, accompanied by obvious fibrosis. The progress of liver fibrosis is obviously related to the increase of acidic procollagen peptides in serum.

　　[Model Evaluation and Application] It is suitable for studying the anti-liver fibrosis effect of drugs and is an ideal model for screening biochemical indicators.