动物造模,胶质瘤原位移植瘤模型 z-bo 2021-01-11 344

　　Objective: To establish a stable nude mouse model of glioma in situ that can be monitored in real time.

　　Method: U251 glioma cells were infected with lentivirus containing luciferase-Luc and green fluorescent protein-GFP genes, and cell lines stably expressing GFP-Luc fluorescence were screened by flow cytometry, and then fluorescent cells were isolated. I passed CCK-8. Experiments to evaluate whether the proliferation, migration and invasion capabilities have changed, cell cycle experiments, transwell tumor migration and invasion experiments; nude mouse tail to establish orthotopic glioma transplantation. The mouse real-time imaging system was used to inject the tumor into the cell nucleus to monitor the brain, so that the tumor can grow. The pathological characteristics and tumorigenicity of naked stone brain cells have been evaluated by paraffin sections and HE staining.

　　Result: We successfully constructed an animal model and U251 glioma cell line stably expressing GFP fluorescence and luciferase fluorescence. Lentiviral integration does not change the ability of cells to grow, migrate or invade. This model has a moderate growth cycle, is highly tumorigenic, and the tumor is a tumor. The human body grows stably in the skull, and HE slices are consistent with the characteristics of human glioma. Conclusion: Compared with traditional cells, dual-fluorescence-labeled glioma cells further promote the experimental study of glioma animal models; U251-GFP-Luc glioma cell nude mouse model has tumor growth similar to humans Its pathological characteristics are similar to those of sarcoma, and the real-time observation of tumor growth makes it an ideal animal model for experimental research of glioma. .. can be used as. \r\nObjective: To establish a stable orthotopic glioma nude mouse model that can be monitored in real time. Method: U251 glioma cells were infected with lentivirus containing luciferase-Luc and green fluorescent protein-GFP genes, and cell lines stably expressing GFP-Luc fluorescence were screened by flow cytometry, and then the fluorescent cells were passed through CCK-8 Experiments, cell cycle experiments, transwell tumor migration and invasion experiments to evaluate whether the proliferation, migration and invasion capabilities have changed; nude mouse tails to establish orthotopic glioma transplantation. Use a mouse real-time imaging system that inoculates the nucleus into the nucleus to monitor the brain The tumors grew, and the pathological features and tumorigenicity of cells in the naked brain of mice were evaluated by paraffin section and HE staining. Make a tumor model. Results: We have successfully constructed an animal model and a U251 glioma cell line stably expressing GFP fluorescence and luciferase fluorescence. Lentiviral integration does not change the ability of cells to grow, migrate or invade. This model has a moderate growth cycle, is highly tumorigenic, and the tumor is a tumor. The human body grows stably in the skull, and HE slices are consistent with the characteristics of human glioma.

　　Conclusion: Compared with traditional cells, dual-fluorescence-labeled glioma cells further promote the experimental study of glioma animal models; U251-GFP-Luc glioma cell nude mouse model has tumors similar to humans The growth and pathological characteristics are similar to those of sarcoma, and tumor growth can be observed in real time, making it an ideal animal model for experimental research on glioma.