动物造模,肝郁证动物模型 z-bo 2021-01-11 335

　　Objective: To compare the effects of single and combined emotional stimulation on animal models of liver depression syndrome, so as to screen out modeling methods similar to clinical liver depression syndrome.

　　Method: Preliminary inspection established a single model of the tail clamp method, the rim binding method and the yoke method, and the three-method composite model. Establish a syndrome comparison table for liver depression and rat syndromes and a syndrome classification table for liver depression rats to determine the TCM syndrome indicators in the body, general observation, body weight indicators, positive drug pair syndromes and hormone indicators are combined to verify Modeling results.

　　Result: The rats in the normal group grew well. The rats in the model group mainly manifested as acute stress mania and chronic stress depression, dry stools, few, few, and most of them had dark red eyes, hair loss and shortness of breath. Rat hypothalamus weight loss, CRH, plasma CORT and ACTH levels were significantly higher than normal control group, and the combined model was more effective than the single model. The positive drug group was treated with drugs. The symptoms were significantly improved, and all indicators were close to the normal group.

　　 Conclusion: The use of tail clamp, limb connection and neck yoke compound emotional modeling method to establish a rat model of hepatic depression syndrome has the characteristics of short cycle, high stability and high success rate. The performance of model animals is related to clinical liver depression. Higher consistency.