动物造模,大鼠动脉移植模型 z-bo 2021-11-11 341

　　Purpose: Allograft vascular disease (AV) limits the function and long-term survival of transplanted organs. This is the most pressing global issue in the field of organ transplantation. Traditional vascular suture requires good microsurgical techniques and is difficult to perform. Exploring more convenient and feasible arterial transplantation methods can provide ideas for clinical research on atrial fibrillation.

　　Method: Use an intravenous needle to make a self-made vascular cannula, take out the thoracic aorta of the donor rat, pull it into the cannula, turn both ends upside down, ligate, and then fix the implant on the recipient On the abdominal aorta of the mouse. Then, one week, four weeks, and eight weeks after modeling, the grafted artery was used for HE staining to observe the morphology of the grafted blood vessel and measure the thickness of the intima.

　　Result: 38 of the 40 recipient rats survived to the required detection point, and by HE staining, the lower limbs and tail, normal urine and urine, and the intima of the transplanted blood vessel moved freely. The display shows concentric circles matching the AV.

　　Conclusion: This method of arterial transplantation to rats is simple and feasible. Compared with traditional vascular suture, it significantly reduces the occlusion time of the abdominal aorta, reduces the surgical trauma to the receiving rat, has a higher modeling success rate, and has a high repeatability.