动物造模,哮喘小鼠模型 z-bo 2021-10-27 274

　　Objective: To observe the role of interleukin 22 (IL-22) in asthma models, and to study the airway inflammation and budesonide's regulatory effects on IL-22 in asthma models.

　　 Method: 24 BALB/c mice were randomly divided into three groups: control group, asthma model group and budesonide group, and the mice were sensitized and stimulated with ovalbumin (OVA) to create an asthma model. .. Mouse lung tissues were stained with HE and AB-PAS to record airway inflammation, the level of IL-22 in mouse alveolar lavage fluid (BALF) was detected by ELISA, and real-time fluorescent quantitative PCR was used. IL was detected in mouse lung tissue. -22 mRNA expression level.

　　Result: Compared with the control group, the lung tissue inflammation score of asthmatic mice increased, the BALF IL-22 level increased, and the lung tissue IL-22 mRNA level increased. This difference is statistically significant. After budesonide treatment, the airway inflammation score and IL-22 mRNA expression in the lung tissue of the mice were lower than those in the asthma group, and the difference was statistically significant.

　　 Conclusion: The therapeutic effect of budesonide on airway inflammation in asthma is related to the inhibition of IL-22 expression and lung secretion.