动物造模,种植性肝癌 z-bo 2022-02-02 442

　　[Modeling mechanism] A liver cancer model formed by transplanting animal or human liver cancer tissues, cell lines and other malignant tumors into animals.

　　[Modeling method] The transplantation site commonly used in this model is the subcutaneous part of the back, liver, and abdominal cavity. The transplantation methods are mainly allogeneic transplantation and xenotransplantation. Rats: The only grafts in this model are allogeneic grafts, BERH-2 transplanted rats and Walker-256 rats with spontaneous hepatic sarcoma. The construction method is mainly to carry out large-scale tumor transplantation under the liver capsule, or intrahepatic injection of cell homogenate. Choose the left lobe of the liver as the injection site, slightly close to the edge of the liver 1-2 cm. Care must be taken to ensure adequate hemostasis after transplantation. Nude mice: Direct inoculation of human liver cancer cell lines (such as Huh7) or cancer tissues into nude mice is xenotransplantation. Common implantation sites are intrahepatic, subcutaneous and peritoneal transplants. Transplantation methods include tissue block transplantation, suspension transplantation and tissue homogeneous transplantation. Mice: BALB/c mice were anesthetized with pentobarbital, and then 20μl in situ inoculated with mouse H22 liver cancer cell line 1x10,000,000 cells/ml. Transplantable rabbit VX2 liver cancer model: The VX2 tumor cell line is derived from the rabbit papilloma squamous cell carcinoma induced by the virus in the workshop, and it is formally established after 72 passages. The VX2 tumor tissue block is made into a cell suspension, and about 1 ml is injected into the medial thigh muscle of the rabbit. After 3 weeks, the tumor-bearing rabbit is under general anesthesia, and the tumor tissue is peeled off to make the diameter of the tumor tissue as large as 1-2 mm. I deleted the general anesthesia for the rabbit, opened the abdomen with ophthalmic forceps, punctured the liver tissue in the thicker part of the central lobe of the left liver, and opened the small mouth (3-5 mm) and outsole (5-8 mm) . Formation of sinuses. Transplant the ruptured tumor line and fill the mouth with a piece of gelatin sponge.

　　[Model Features] The transplanted rat liver cancer model has simple animal breeding, simple transplantation methods, high transplant success rate, fast tumor growth, short survival time of mice, and stable and uniform tumor biological characteristics. , And has similar benefits as primitive human blood donor liver cancer. The cancerous tissue in the transplanted abdominal cavity grows up, mainly between the liver and the stomach, and cancerous ascites may appear later. Subcutaneous tumor implantation is shallow and limited. Tumors inoculated by intrahepatic transplantation are round or oval in the liver, swelling and infiltrating, and the incidence of ascites is high. The nude mouse transplantation model retains the morphology, function and secretion of alpha-fetoprotein (AFP) characteristics of human liver cancer. Pathologically, in the rabbit VX2 implantation model, the tumor is a large solid tumor with aggressive growth and abundant blood supply, similar to large liver cancer.

　　[Model Evaluation and Application] Rat implant models and VX2 implant models are often used in imaging experiments and local intervention treatments. In addition, subcutaneous implantation in rats has a high spontaneous regression rate and is usually used to study the mechanism of spontaneous tumor regression. Intrahepatic transplantation is an excellent model for studying local treatment of liver cancer. Nude mice are usually used to study the biological characteristics and etiology of liver cancer.