动物造模,鼠肠道模型 z-bo 2021-01-11 431

　　Objective: To establish an intestinal model of Salmonella typhimurium infection in murine C57BL/6 mice.

　　 Method: First, add 5% LVDS3 solution to the stomach to neutralize some gastric acid and increase the infectivity of Salmonella typhimurium, and then use Salmonella typhimurium to attack the stomach. Observe the clinical symptoms of the mice, perform statistical analysis on the survival ability and weight of the mice, and analyze the pathological changes of the mouse intestinal tissue through histopathological sections.

　　Result: C57BL/6 mice showed weight loss, disease, cold and death after Salmonella typhimurium challenge, and the anatomical level showed intestinal congestion and swelling of the mice. Pathological analysis showed edema, necrosis of the intestinal mucosa and infiltration of inflammatory cells in the mouse small intestine.

　　 Conclusion: Salmonella typhimurium is infected with Salmonella typhimurium C57BL/6. We have successfully established a mouse intestinal model. This model is S. This is very important in the study of the biological function and the treatment of related immune molecules or cytokines in the process of murine typhoid enteritis. It has scientific significance.