动物造模,子宫内膜异位症盆腔粘连大鼠模型 z-bo 2021-01-12 338

　　Objective: To explore the method and evaluation index of establishing a rat model of endometriosis with pelvic adhesions.

　　 Method: The endometrium was implanted into the mesentery of rats by self-implantation technology, and 8 rats were randomly transplanted on the 1, 3, 5, 7, 14, 21 and 28 days after the operation. We selected and observed the growth of the lesion. The degree of pelvic adhesion was scored using the Association for Reproductive Society (AFS) adhesion rating (AFS) and the harbor adhesion score, and the lesions and surrounding mucosal and peritoneal tissues in each group were used for HE staining; 5 points after surgery. On 7, 14, 21, and 28 days, the adherent tissues around the lesions in the group were collected, and the histological plasminogen activator (tPA) content was dynamically detected.

　　Result: The two bond scoring methods both show that the typical pelvic bond is formed 5 days after modeling. Compared with the blank control group and the sham operation, the tPA content of the pelvic mucosal and peritoneal tissues in each model group was significantly reduced after 5 days of modeling. (P\u003c0.01), 28 days after modeling, the tPA content showed an upward trend, but it was still significantly lower than the blank control group and the sham operation group (P\u003c0.01).

　　 Conclusion: The EMs pelvic adhesion model is established by autotransplanting the endometrium into the mesentery. The mature model is 5 days after modeling. There is a correlation between tPA and EMs pelvic adhesions.