动物造模,不同感染途径致大鼠肺炎模型 z-bo 2021-01-12 599

　　Objective: To explore the establishment of a rat model of pneumonia caused by Pseudomonas aeruginosa, and to lay the foundation for subsequent experiments.

　　 Method: 48 Sprague-Dawley (SD) rats were randomly divided into 4 groups: control group, B tracheal injection group, C tracheal intubation infection group, and D nasal drip infection group. After adapting to eating for 3 days, on the 5th, 10th and 15th days after the intervention, various model interventions were carried out, including rat body weight, body temperature, white blood cell count and pathological changes of lung tissue in each subgroup. Be dynamically monitored.

　　 Results: The behavioral symptoms, weight, body temperature, white blood cells, lung inflammation and pathological symptoms of rats in each model group were significantly different from those of the control group; ②Each model group was infected with Pseudomonas aeruginosa, that is, the control group. After confirmed as negative.

　　 Conclusion: Infection of Pseudomonas aeruginosa through the nasal route can provide a qualified rat pneumonia model. This transmission route avoids interference with the inflammatory response caused by surgical wounds, is simple, easy to implement, and can be facilitated.