动物造模,不同性别氟中毒大鼠模型 z-bo 2021-12-02 324

　　Purpose: Do you want to establish an animal model of male and female fluorosis rats to study the sex differences in fluorosis?

　　Method: Select 48 SD rats weighing about 150 g and divide them into a control group and a fluoride exposure group. The model group can freely consume fluoride-containing feed. The fluoride content in the feed is 100 mg/kg, and its condition, daily changes in body weight and tooth fluoride content are dynamically observed every 3 days. When dental fluorosis occurs in all female and 110-day male rats, blood will be drawn from the femoral artery. Is a batch of rats using fluoride ion selective electrode method to detect fluoride in urine and fluoride in bone?

　　Result: Did the male and female rats in the control group show dental fluorosis throughout the experiment? Male and female rats in the fluoride exposure group first developed severe fluorosis about 60 and 80 days after fluoride exposure. Compared with female rats, the first appearance of fluorosis in the teeth of male rats was delayed by about 20 days. About 30 days after exposure to fluoride, male and female rats entered a rapid growth phase, and the weight difference between female and female rats was significant (P\u003c0.01)? Are the male and female rats in the 110-day fluoride exposure group heavier than the control group due to long-term fluoride exposure? With the prolongation of fluorine exposure time, the urine fluoride and bone fluoride of male and female rats gradually increased, and the fluoride exposure group was significantly higher than the control group. Significantly (P\u003c0.01), is there a positive correlation between fluoride urine and fluoride bone and fluoride exposure time? The content of fluoride urine and bone fluoride in male rats was higher than that in females, and the difference was statistically significant (P\u003c0.01). Gender influence?

　　 Conclusion: What are the body weights of male and female rat models of fluorosis? When does fluorosis appear? Are there gender differences in the levels of fluoride in urine and bones?