动物造模,大鼠宫内发育迟缓模型 z-bo 2021-01-12 316

　　Purpose: Is intrauterine growth retardation (IUGR) manifested in the form of low birth weight? The IUGR model was established through three commonly used experimental methods, and the IUGR model in scientific research was established through the comparison and analysis of experimental data. Do you provide reference standards?

　　Method: Divide 20 pregnant mice on the 0th day of pregnancy into 4 groups randomly, 5 in each group, 3 of which received alcohol intervention (A)? Uterine artery ligation (U)? Low IUGR rat models have been established through protein diet (LP) and other methods. The fourth group is a normal control group (CON). The fetuses were harvested through royal incisions on the 20th day of pregnancy. We measured the weight of fetal mice and the mass of their brains. It measures body length and tail length (such as placental weight and kidney weight), and calculates the incidence of IUGR, survival rate, brain weight, and placental weight. Did each group continue to undergo a royal fetal resection randomly within 3 to 6 weeks? Do you want to weigh within 12 weeks? Kidney fat mass and some organ mass?

　　 Results: After 12 weeks of rearing, the average weight of the three groups of fetal mice with alcohol intervention, uterine artery ligation and low-protein diet was significantly lower than that of the normal control group (P0.05). , The average weight of rats in the alcohol intervention group and the low-protein diet group was significantly higher than that of the normal control group (P\u003c0.05)?

　　 Conclusion: All three modeling methods can be used to build the IUGR model. Among them, the IUGR animal model established by the low-protein diet group has a high incidence and a low birth rate, which is suitable for model research. From the perspective of catching up with growth, Cheesys worm rats used alcohol intervention group and low-protein diet group during postpartum and lactation. The average weight is lower than the normal control group, but the growth rate after breastfeeding is much higher than the normal control group. Does this indicate that the two experimental groups are catching up with growth?