动物造模,兔卵巢早衰动物模型 z-bo 2021-01-12 411

　　Objective: To compare different injection methods to establish a rabbit model of early ovarian failure induced by cyclophosphamide, find the best modeling method, and provide a more suitable experimental model for further research on early ovarian failure.

　　 Method: 21 rabbits were randomly divided into 4 groups. Three are the conventional control group (group A) without any treatment. In the model group (group B), six animals were treated with 50 mg/kg cyclophosphamide solution for 1 day. 2d (group C); 6 rats in the 3 groups of models were injected with 50 mg/kg on the first day and then with 8 mg/kg for 14 consecutive days (group D)? Weigh the rabbits and ovaries in each group, explain the body weight, change, and analyze the ovarian index within 14 days after injection; HE staining to observe the shape of the follicles, and observe the number of follicles at various levels under an optical microscope. Calculation; TUNEL method to observe and analyze cell apoptosis; ELISA method to analyze changes in serum estradiol (E2)?

　　Result: Weight: B? There was no significant change in body weight in group C (P\→0.05); growth restriction of rabbits in group D (P0.017)? One kind? B? There was no significant difference in the rate of secondary antral follicle abnormalities among the three groups C (P\→0.05). Apoptosis rate: Apoptosis mainly occurs in follicular granuloma cells. The apoptosis rate of group B and C is significantly higher than that of group A (P\u003c0.05), and the rate of apoptosis of C group is higher than that of B group. Very high (P\u003c0.05). 0.05) Serum E2 level: E2 secretion in group B reached its peak on the 7th day, and gradually decreased after 7 days, and E2 secretion on the 7th day was significantly higher than that on the 35th day (P\u003c0.05). ); The E2 value of group C continued to decline on day 0. Is significantly higher than the 35th day (P\u003c0.05)?

　　 Conclusion: Is 50 mg/kg continuous injection for 2 days the best way to establish a rabbit model of early ovarian failure induced by cyclophosphamide?