动物造模,树鼩胆石模型 z-bio 2021-01-13 398

　　Objective: To establish a reasonable and stable mouse model of hyperuricemia nephropathy, and provide a pathological model for screening and researching drugs for the treatment of hyperuricemia nephropathy? Method: potassium oxalate? Xanthine? Are you still there? Do you only use 5 styling agents for Etambutor and YeastCream? Do you use a combination of two drugs or a combination of three drugs? Do you observe different modeling times? Modeling dose and modeling method. Have you established a serum kidney injury model for uric acid, urea nitrogen and creatinine levels in hyperuricemia mice? Changes in the activity of liver xanthine oxidase (XOD) and adenosine deaminase (ADA)? What are the pathology and weight changes of each group of kidneys?

　　Result: When model mice were combined with xanthine and oxazine potassium, their serum uric acid and urea nitrogen levels were significantly increased (P\u003c0.01). Compared with the normal group, the renal tubular casting was obvious. .. Salt crystals were found in the renal cortex and medulla of mice in the 7d group; when combined with hypoxanthine, eptificol, and potassium oxalate, serum uric acid levels and urea nitrogen were normal, while XOD activity in the gyratory liver was significant. Is increasing. (P \u003cu\→ u003c0.01), liver XOD activity was significantly reduced (P \u003c\u003c u003c0.05), renal eosinophil insoluble protein and yeast extract, potassium oxalate 14d group and adenine yeast extract Compared with the 14-day potassium oxalate group, the serum uric acid, urea nitrogen and creatinine levels of mice were significantly increased (P \u003cu003c0.01). In the yeast extract and potassium oxalate groups, renal tubular epithelial cells were shed, and eosinophil insoluble proteins were found in the proximal rotation of the kidney, yeast extract, adenine, and potassium oxalate. The kidney medulla of this group of mice has salt crystals, the combination of yeast cream and potassium oxalate gains weight faster than the combination of body weight with yeast cream, adenine and potassium oxalate, and the difference between the two groups is significant.

　　 Conclusion: Compared with other modeling methods, the hyperuricemia kidney injury mouse model established by the combination of yeast extract and potassium oxalate is more stable and has a significant impact on the weight of the mice. Do not do. At the same time, because the modeling method is more in line with clinical characteristics, is it more appropriate to use yeast cream and potassium oxalate to establish a 14-day mouse model of hyperuricemia nephropathy? Purpose: How to study the relationship between the Tree Schlugal Stone model and P(SP) substances established by feeding Tree Schrew's high cholesterol diet?

　　 Method: Detect the expression of SP immunohistochemistry in the gastrointestinal tract by establishing a tree-like shrug stone model. Comparative analysis of the control group? Results: Compared with the control group, the immunohistochemical expression level of SP in the gastrointestinal tract of the tree shrub was lower than that of the control group (P\u003c0.05), while the SP expression level of the tree tower model was negative. Is it relevant?

　　 Conclusion: Is the tree shrug Blood stone related to the regulation mechanism of gastrointestinal hormone secretion?