肝脏肿瘤动物模型,动物造模 z-bio 2021-01-13 485

　　Objective: To establish a new animal model of advanced liver tumors by injecting tumor cells into the portal vein.

　　Method: Human hepatocellular carcinoma obtained by culturing MHCC97? H? Human colorectal cancer SW480? Human non-small cell lung cancer A549 and human triple negative breast cancer MDA? MB? These malignant tumor cells 231 and other cell lines were injected from the pulmonary portal vein of BALB/c nude mice. Liver after 3-4 weeks of growth, PET/CT test: about 200μCi nuclear probe 18F? Inject FDG into the tail vein, and then perform PET/CT testing with BALB/c nude mice 30-40 minutes later? Based on this, do you want to collect animals to obtain livers and take photos to identify tumor lesions in the liver of BALB/c nude mice?

　　Result: MHCC97? H? SW480? A549 and MDA? MB? BALB/c nude mouse liver may form multiple diffuse tumor lesions with 231 cells PET/CT can detect tumor cell proliferation in nude mouse liver SW480, MDA, MB, 231, A549 lesions are formed by cells MHCC97? It has a weaker PET/CT detection signal than H.

　　 Conclusion: Have you successfully obtained a new animal model of advanced liver tumors and laid a solid foundation for screening drugs related to advanced liver tumors?