小鼠谵妄模型,动物造模 z-bio 2021-01-14 312

　　OBJECTIVE: To use combinatorial behavioral experiment to detect scopolamine to make a mouse model of delirium.

　　Methods: The behavioral changes of the two groups of mice were compared: mice in the delirium group (n=12) and control group (n=8) were injected intraperitoneally with 15 mg/kg scopolamine and an equal volume of normal saline, 30 to 60 minutes after injection The two groups of mice were sequentially subjected to a black and white box experiment for 10 minutes, an open field experiment for 10 minutes, and a non-selective non-continuous attention experiment for 10 minutes—combined behavior experiment method to compare the five behavioral indicators of the two groups of mice to detect delirium.

　　Results: Compared with the control group, the five behavioral indexes of the mice in the delirium group were significantly different. The time that the mice stayed in the white box was significantly shorter, P<0.05; the activity speed of the mice was significantly increased, P< 0.0 Significant increase in activity time along the wall, P<0.0, significant reduction in freezing time, P<0.05; The level of attention in mice decreased significantly, P<0.05.

　　Conclusion: The combined behavior experiment method can reflect the diagnostic elements of delirium, better reflect the state of delirium, and it takes a short time.