食管癌细胞动物模型,动物造模 z-bio 2021-01-14 276

　　Objective: To establish a green fluorescent protein (GFP)/luciferase (Luc) dual-labeled human esophageal cancer ECA109 cell line (ECA109-LucGFP), and use non-invasive in vivo imaging technology to treat esophageal cancer in nude mice. How to monitor the tumor model?

　　Method: MTT? Flow cytometer? Scratch test analysis to detect the biological characteristics of ECA109-Luc-GFP cell line and normal ECA109 cells? Do you use an in vivo imaging system to track tumors?

　　Result: Compared with normal ECA109, ECA109-Luc-GFP cells have no significant difference in cell viability, proliferation and migration ability (P≥0.05)? Are biological characteristics such as tumor growth, metastasis, etc. observed in the in vivo imaging experiments of small animals?

　　 Conclusion: The GFP/Luc dual-labeled human esophageal cancer ECA109 cell line (ECA109-Luc-GFP) was successfully constructed, and the application technology of imaging in small animals was applied, and the growth of esophageal cancer animal models was observed in vivo.