动物造模,乳腺炎动物模型 z-bio 2021-11-17 292

　　OBJECTIVE: To study the effect of glycosides on the expression of IL-6, TNF-α and IL-1β in mammary gland tissue and breast epithelial cells of mice with mastitis.

　　Method: 40 postpartum female mice were randomly divided into a blank control group. 12 hours after modeling, whether groups L, M and H, low-dose protoside treatment group L, middle-dose protoside treatment group M, high dose protoside treatment group H, blank control group and model group were given the same Amount of phosphate buffered saline? After 24 hours of modeling, have you observed the effect of the intervention?

　　Result: Model group? L group? TNF-α in group M and H? IL-1β? The level of IL-6 mRNA was significantly higher than that of the blank control group (P \u003cu003c0.05); TNF-α in the L, M and H groups? IL-1β? The level of IL-6 mRNA is the model group (P\u003c0.05); the M and H groups are TNFα. IL-1β? It is significantly lower than IL. -6 mRNA level was significantly lower than that of L group (P\u003c0.05); TNF-α, IL-1β and IL-6 mRNA levels of H group were in M and L groups (P\u003c0). 05) The protein levels of TNF-α, IL-1β and IL-6 were significantly lower than those of the L group, and the M and H groups were significantly lower than the model group (P\u003c0.05). The protein levels of TNF-α, IL-1β and IL-6 in group M and H were significantly lower than those in group L (P\u003c0.05). 0.05); TNF-α in group H? IL-1β? The protein level of IL-6 was significantly lower than that of the M and L groups (P\u003c0.05)?

　　 Conclusion: Gen glycosides can prevent the inflammatory damage of the breast caused by lipopolysaccharide. Did you reduce the levels of TNF-α, IL-1β and IL-6 inflammatory factors?