动物造模,SD大鼠肢体缺血-再灌注损伤模型 z-bio 2021-01-14 300

　　Objective: To introduce an improved method of blocking and blocking femoral blood flow in vitro, and to establish a simple, controllable and reliable rat model of limb ischemia-reperfusion injury.

　　Methods: Fifteen 2-month-old male SPF male SD rats were randomly divided into 5 groups: normal group, ischemia-reperfusion 0 hour group, 2 hour group, 4 hour group, 8 hour group, normal group does not block blood flow The remaining 4 groups were models of ischemia-reperfusion injury. The morphological changes of skeletal muscle cells were observed by HE staining and apoptosis was observed by DAPI.

　　Result: Except for the normal group, each group showed different degrees of muscle fiber swelling, irregular placement and enlarged muscle fiber gap. In other cases, the injury in the 4h and 8h groups was significantly more severe than that in the 0h and 2h groups, the 8h group showed the most severe injury, and the four groups of skeletal muscle cells were typical apoptosis.

　　Conclusion: This method can establish an animal model that reflects limb ischemia-reperfusion, which can simply and effectively reflect the pathological characteristics of skeletal muscle in LIRI and the clinical characteristics of LIRI.