动物造模,大鼠子宫内膜异位症模型 z-bio 2021-01-14 583

　　Objective: How to study the curative effect and recurrence effect of different doses of estrogen in the treatment of endometriosis with GnRH-a?

　　Methods: 51 endometriosis model rats were divided into 5 groups: control group (n = 10), saline 1 mg/kg subcutaneous injection, once; study group: anti-additive group I (low dose estrogen Group n = 10)? Anti-additive group II (medium-dose estrogen group) n = 10)? Anti-additive group III (high-dose estrogen group n = 10)? GnRH-a group (n = 11)? Initially, the study group was injected with a dose of leuprolide 1 mg/kg subcutaneously. In the second week of administration, groups I, II and III were given different doses of estradiol valerate (25μg/kg?50μg/kg?100μg/kg) by gavage, and normal saline was given to the GnRH group. Mandatory oral. 3 mL per mouse, once a day for each study group for 14 days. Abdominal exploration was performed on the 4th and 7th week after administration, the amount of internal abnormalities was measured, and internal abnormal tissues were collected. TNF was detected using immunohistochemical methods. -α? Enzyme-linked immunosorbent assay can detect the expression of StAR, blood samples of the orbital vein flora, and serum CA125 levels? Results: Before treatment, there was no significant difference in the volume of ectopic lesions in each group of rats at the 4th week ((P<0.05), the study group was significantly smaller than the control group, the difference was statistically significant. The difference was statistically significant (P0. 05); At the 4th week, the expression of TNF-α? StAR in the study group was significantly lower than that of the control group; significant (P0.05)?; At the 4th week, the expression of the study group was significant (P0.05). CA125 was lower than the control group, the difference was statistically significant (P\u003c0.05). In the 7th week, the serum CA125 levels of the anti-additive treatment group II and A group were higher, and the difference was statistically significant (P\u003c0.05) ). u003c0.05)?

　　Conclusion: Does GnRH reverse low-dose estrogen induce TNF-α? StAR? For the rat endometriosis model, high-dose females reversely adding hormones have a better effect.