动物造模,原位移植瘤模型 z-bio 2021-01-14 569

　　Purpose: Do you want to select 3 human liver cancer cell lines and inject them into the liver tissues of 4 different immunodeficiency mice to establish and compare orthotopic transplanted human liver cancer models?

　　Method: Inoculate the human HepG2 HUH-7 and QGY-7703 cell suspensions into the liver tissues of mice with different immunodeficiencies (BALB/c nude mice, NODSCID mice, NOG mice, NPG mice). The final statistical model mouse death time? death? Do you want to use liver weight, B-ultrasound and histological examination methods to analyze and compare the characteristics of liver cancer models in various immunodeficiency mice?

　　 Results: B-ultrasound examination and macroscopic anatomical observation showed that mice in each experimental group were tumor nodules in liver tissue and were inoculated with HepG2 cell suspension. All animals in each group died about 20 days after the experiment, which showed that the survival time of NOG and NPG mice was significantly shorter than that of BALB/c nude mice and NODSCID mice (P\u003c0.001); in each experimental group HUH-7 and QGY-7703 were inoculated into the liver of humans, and animals were dissected on the 92nd and 104th days of the experiment. The liver volume of NOG and NPG model mice increased significantly, resulting in huge tumor masses. c nude mice and NODSCID mice showed only small tumor nodules in the liver tissue; the liver weight of NOG and NPG mice inoculated with HUH-7 and QGY7703 was significantly higher than that of BALB/c nude mice and NODSCID mice (P). Histological examination showed that tumor cell growth and extensive necrosis occurred in the liver tissue of each group, and tumor cell metastasis occurred in the lung tissue of some animals. Do OG and NPG mice grow faster in liver tissues and eventually show shorter survival periods, larger livers and weight gain? Since NOG and NPG mice can complete the malignant growth of human liver cancer cells and shorten the model in a relatively short time, orthotopic transplantation of human hepatocellular carcinoma in NOG and NPG mice is the development of anti-liver cancer drugs.