动物造模,薄型子宫内膜小鼠模型 z-bio 2021-01-14 377

　　Purpose: Through immunohistochemistry and fertility evaluation, to further study the possibility of establishing a stable thin endometrial mouse model by injecting 95% ethanol into the uterine cavity, and the pathological characteristics of the thin endometrial layer. Provide an ideal animal model for research on repair mechanisms?

　　Method: By selecting 78 female C57BL/6J mice with normal estrus as experimental animals, and injecting 95% ethanol into the uterine cavity to damage the endometrium, thereby making the uterus thin. Do you want to establish an intimal mouse model? 78 experimental mice were randomly divided into two groups, 30 in each group, randomly divided into blank group, control group and experimental group. In the third estrus period after modeling, experimental mice were harvested at the expense of cervical dislocation. Immunohistochemical method was used to detect the expression of cytokeratin, vimentin, vascular endothelial growth factor and estrogen receptor α to evaluate the regeneration of uterine samples and endometrial cells; 48 independent groups were randomly divided into control groups Group, unilateral injury group, bilateral injury group. After modeling, all surviving mice were mated in the third estrus to analyze the effect of thin endometrium on the fertility of mice, and evaluate the mice with thin endometrium.

　　 Results: The results of immunohistochemistry showed that cytokeratin, vimentin, vascular endothelial growth factor and estrogen receptor α were significantly expressed in experimental mice injected with 95% ethanol into the uterine cavity. Is it lower than the blank group and the control group? Fertility evaluation experiments showed that after cage mating, blank mice and control mice were successfully pregnant, mice that were injured on both sides were not pregnant, and the uterus of the experimental group that was injured on one side were not pregnant. it is. The injured uterus was conceived normally, and the average pregnancy rate of the injured uterus was significantly lower than that of the intact side (P\u003c0.05), and injection of 95% ethanol into the uterine cavity would damage the endometrium of mice.

　　 Conclusion: Has this study successfully established a thin endometrial mouse model that can be used to study thin endometrial repair and repair mechanisms?