动物造模,高脂饮食诱导果蝇心衰模型 z-bio 2021-01-14 346

　　Objective: To establish a model of heart failure induced by high-fat diet in Drosophila. Method: Choose wild fruit fly w1118 fruit flies as the experimental target, and use various concentrations of coconut oil (0%, 7.5%, 15%, 30%) to feed the fruit flies for 5 days. Do you want to test the triacylglycerol of fruit flies? content? In the follow-up experiment, did you use the non-high-fat feeding group (0% coconut oil) as the control group and the 30% coconut oil high-fat feeding group as the experimental group? Does the negative geotaxis test detect the performance of fruit flies? Drosophila adult heart function analysis platform, under semi-anatomical conditions (30s), take images of the heartbeat of fruit flies to obtain adult heart function indicators, such as heartbeat cycle, systolic and diastolic diameters, and cardiac contractility software analysis indicators? Real-time quantitative PCR to detect changes in RNA expression in the heart of fruit flies on a high-fat diet?

　　Results: Compared with high-fat flies, the concentration of coconut oil in high-fat flies increased according to different proportions of triacylglycerol content (P\u003c0.05); high-fat flies diet greatly affected motility (P\u003c0) .001); shorten the heartbeat cycle of high-fat-fed flies (P\u003c0.01) and weaken the contractility of the heart (P\u003c0.001); real-time quantitative PCR has found several factors in the lipid metabolism of high-fat flies The expression of two genes fa2h, CG6277, CG3699, CG9914 and lip2 was significantly down-regulated (P\u003c0). .05)?

　　Conclusion: High-fat diet has a serious impact on the exercise performance of fruit flies and the heart of adults. Have you successfully established a functional, high-fat diet-induced Drosophila heart failure model?