动物造模,小鼠体力疲劳模型 z-bio 2021-01-14 353

　　Objective: To study the physical fatigue of mice caused by sleep interruption (SI) at different times, and to provide a stable and reliable animal model for the development of products that reduce physical fatigue.

　　Method: Use drum type sleep disturbance devices (5, 10, 15d) to perform different periods. After sleep disorders, run and load-bearing swimming experiments were performed on the liver of each group of animals and mice. Have you measured the levels of glycogen and serum urea nitrogen?

　　Result: In the running experiment, sleep disturbance was 5-10d. Compared with the control group, the 15th day group showed a downward trend in time, but the difference was not significant (P\→0.05). Compared with the blank control group, sleep disturbance was 10 days, and the fatigue time of weight-bearing swimming was significantly reduced (P\u003c0.05, P\u003c0.05). 0.01), the number of receivers and the total receiving time are significantly reduced (P\u003c0.0P\u003c0.01). Compared with the control group, the hepatic glycogen content of the interference 5d, 10d and 15d groups was significantly reduced (P\u003c0.05); the interference was 10d-15d. Compared with the control group, whether the serum urea nitrogen content increased significantly (P\u003c0.05).

　　 Conclusion: Does the roller method sleep disorder (rolling parameter 3r/min, 1 minute per revolution, 1 minute interval) 10 to 15 days will cause physical fatigue behavior in mice?