动物造模,口腔黏膜炎模型 z-bio 2021-01-14 334

　　Objective: How to establish a golden hamster oral mucositis model combining 5-fluorouracil and mechanical scratches.

　　Method: The male golden hamsters were divided into a control group and a model group, and the animals in the model group were injected intraperitoneally on the first day and the second day. 60mg/kg-40mg/kg 5-Fluorouracil (5-Fluorouracil, 5FU), on the 4th day, turn the golden hamster's right cheek pouch over, and use an 18-gauge needle on the surface for linear scraping Will you form an oral cavity? ? The animals in the control group were injected intraperitoneally with the same dose of normal saline on the first and second days. After modeling, observe the daily macroscopic changes of the right cheek pouches of each group of animals, and take pictures to record the weight and average daily food intake of each animal. Blood was collected on the 8th and 14th days after the model was established, and the levels of serum inflammation-inducing cytokines TNF-α and IL-1β were measured, and the right cheek pouch tissue was collected for HE staining. Maleic dialdehyde (MDA) was measured.

　　Result: Compared with the control group, the golden hamsters in the model group had ulcers in the right cheek pouch tissue and had the highest macroscopic score 3 days after modeling. On the 8th and 14th days after modeling, the average daily intake and body weight of the animals decreased significantly within 8 days after modeling. The structure of the golden hamster's right cheek pocket was damaged and inflammation occurred. Show serum TNF-α? Is there a significant increase in IL-1βMDA content, a significant increase in MDA content in cheek pouch tissue, and a significant decrease in SOD activity?

　　Conclusion: 8 days after modeling, combining 5-fluorouracil with mechanical scratches will cause golden hamsters to form oral mucositis. The internal inflammatory reaction is obvious, and it is most obvious on the 3rd day. It can be used to study oral mucositis and The cause of the medication.