动物造模,子宫内膜异位症模型大鼠 z-bio 2021-01-14 325

　　Objective: To explore the effect of Wenjing Zhitong Recipe on the expression of AKT/mTOR pathway related molecules in endometriosis model rats.

　　Method: The 75 mature Wistar rats successfully modeled were randomly divided into two parts. In addition to the 5 groups, there are a total of 6 groups, namely the sham operation group, each with 15 rats. Give them high, medium and low doses of warming and pain relief prescriptions, Nimetone (progesterone) capsules, normal saline and intragastric administration. TimePCR? Does immunohistochemistry detect ectopic? Upright endometrium AKT? mTOR mRNA and protein expression? ELISA method was used to detect serum vascular endothelial growth factor (VEGF) content.

　　Result: The contents of AKT and mTOR mRNA and their protein in the ectopic endometrium of endometriosis model mice were significantly higher than those in the normal control group (P\u003c0.01). The treatment of ectopic endometrial AKT with high-dose Chinese medicine? Western medicine (guest) group? The expression level of mTOR mRNA was significantly lower than the model (P\u003c0.05). The serum VEGF level of the EMS model group was higher than that of the normal control group (P\u003c0.01). Significantly lower than the model group (P\u003c0.01); whether the traditional Chinese herbal medicine administration group is also lower than the model group (P\u003c0.05).

　　 Conclusion: Is the occurrence of endometriosis related to the AKT/mTOR signaling pathway and the expression level of VEGF? Does Wenjing Zhitong Decoction effectively block the AKT/mTOR signaling pathway and reduce serum VEGF expression, thereby inhibiting ectopic endometrial adhesion, invasion and angiogenesis?