动物造模,诺如病毒,树鼩小动物模型 z-bio 2021-01-14 387

　　Objective: How to explore the possibility of establishing a tree shrew infection model through garbage challenge.

　　 Method: Put two groups of 6-month-old tree cages into GII.4 HuNoVs2010 and HuNoV respectively. The stool suspension of the Sydney strain was observed for 7 consecutive days after the challenge. The control group was filled with the same amount of PBS (5 in each group), signs and diarrhea were observed, HuNoVsRNA was detected by RT-PCR, and stool tissue and trees were collected every 24 hours The small intestine collected after killing, the pathological changes observed by HE staining, the identification of virus antigen and human tissue blood group antigen (HBGA) by immunohistochemistry, and the analysis of potato saliva

　　Result: Tudou did not appear after being attacked by HuNoV. Obvious symptoms of infection and diarrhea, HuNoVsRNA was not detected in stool samples, HuNoVs antigen was not detected in small intestine tissue, pathological changes were not obvious, HBGAs antigen in squirrel saliva was negative, are you?

　　 Conclusion: Tree Shu is not sensitive to HuNoV. The non-secretion and expression of HBGA are related to general reproductive and reproductive status. Whether trees infected by HuNoVs need to be further studied in small animal models.