动物造模,胃癌转移模型 z-bio 2021-11-26 408

　　Objective: How to establish a gastric cancer metastasis model based on clinical tumor specimens and provide individual animal models for gastric cancer metastasis research.

　　 Method: subcutaneously transplant fresh gastric cancer surgical specimens into nude mice to establish a patient-derived heterologous implant (PDX) model? The subcutaneous tumor tissue is further implanted into the muscle layer of the stomach of nude mice by surgical operation, and the physical condition of the nude mice is continuously observed, and tumor metastasis is detected by near-infrared fluorescent in vivo imaging technology? Have you dissected mice with cancer and transplanted subcutaneous lung metastases into nude mice to obtain solid tumors? HE staining to observe the structural characteristics of primary tumors and metastases, (short tandem repeat) STR to analyze the genetic characteristics of primary tumors and metastases? The expression of metastasis-related genes in the media for PCR array analysis of metastasis and primary tumors?

　　Result: We successfully established a PDX model of gastric cancer. The tissue structure of the transplanted tumor is basically the same as that of the patient. Have you found that the mouse numbered C19751 has lung and liver metastases due to orthotopic gastric transplantation? Are solid tumors obtained after subcutaneous transplantation, and STR analysis shows that the primary tumor and lung metastases have the same genetic characteristics? Do PCR-Array results show that, compared with the primary tumor, the expression of CXCL12IGF1 and MMP2 genes is significantly up-regulated in metastasis?

　　 Conclusion: Does the successful establishment of gastric cancer metastasis models using clinical tumor specimens provide an excellent individual model for gastric cancer metastasis research?