动物造模,胰岛素抵抗动脉粥样硬化模型 z-bio 2021-01-15 294

　　Objective: To investigate the phenotypic differences and pathological mechanisms of insulin-resistant atherosclerosis models between white-haired black-eyed rabbits and Japanese big-eared rabbits.

　　Method: Did you divide the WHBE rabbits into a normal control group (NC) and a high-fat and high-sugar diet (HF) group, each with 6 rabbits, and divide them into 12? The IR-AS model was induced with HF diet for 12 weeks. After modeling, collect blood and measure blood lipids. -Superoxide dismutase (SOD) and malondialdehyde (MDA) levels; perform a glucose tolerance test to calculate the area under the blood glucose and insulin curve; detect liver microsomal triglyceride transfer protein (MTTP); nuclear factor E2 ( Nrf2) Have you observed SOD1 gene expression, pathological changes of fat and aortic blood vessels, and CD68 expression in blood vessels by HE staining?

　　 Results: Compared with the NC group, the HF group had obesity, elevated blood lipids, impaired glucose tolerance and hyperinsulinemia, HOMA-IR, decreased plasma and liver SOD activity, and increased MDA content. However, compared with JWHF group, liver MTTP and Nrf2 gene expression increased, SOD1 gene expression decreased, vascular lipid deposition and AS and vascular CD68 expression were significantly increased; WBHF group TG, LDL-C were higher than JWHF group. , HOMA-IR, area under the glucose tolerance curve (U_GLU), MDA content, fat diameter, liver SOD1 gene expression, AS lesion level and vascular CD68 expression are there major differences?

　　 Conclusion: Can a high-fat, high-sugar diet induce the formation of IR-AS in rabbits, thereby showing lipid metabolism, inflammation and AS damage? Is there a difference between lipid metabolism and oxidative stress in rabbit strains?