白血病模型,动物造模 z-bio 2021-01-15 300

　　Purpose: Do you want to use BALB/c nude mice to establish an M1 leukemia model?

　　Method: Twelve female BALB/c nude mice aged 5-6 weeks were randomly divided into low, medium, high dose groups and blank groups. Only three groups of tail vein injections (low, medium and high) were inoculated with log M1 cell suspensions at 1x106, 5x106 and 8x106 respectively. Observe the general condition of the mouse. Collect peripheral blood at 0, 10, 20, and 30 days after modeling to test your blood routine. Perform white blood cell classification. Samples were collected on the 30th day or on death, and flow cytometry was performed to detect the proportion of CD33CD117-positive cells in peripheral blood and bone marrow, and to prepare tissues. Pathological section?

　　Result: 10 to 15 days after vaccination, are the mice in the model group sick, lack of exercise, spinal curvature and other symptoms? On the 30th day of the experiment, the number of peripheral blood leukocytes in the high-dose group was significantly higher than that in the blank group (P\u003c0.05); the proportion of peripheral blood leukemia cells in each group was higher than that in the blank group. It was also significantly higher (P\u003c0.05); the ratio of CD33 + CD117 + in the bone marrow of each group of mice increased, and the high-dose group was the most significant (P\u003c0.05)); whether there was a small amount in the spleen of this group Leukemia cell infiltration?

　　 Conclusion: BALB/cNude mice can be injected with 8×106 mouse leukemia cells M1 through the tail vein to establish an acute myeloid leukemia model consistent with acute myeloid leukemia.