动物造模,人肝癌裸小鼠原位癌模型 z-bio 2021-11-09 370

　　Objective: How to select a human hepatocellular carcinoma cell line (HCCLM3-Luc) stably expressing the luciferase reporter gene and establish an orthotopic tumor human hepatocarcinoma cell line nude mouse model.

　　Method: Quantitatively detect different cell numbers by in vivo imaging of HCCLM3-Luc fluorescence intensity expression, and detect the luciferase activity in each cell number between the number of photons produced by HCCLM3-Luc luciferase and the number of tumor cells. Do you want to study Linear correlation? In situ injection of HCCLM3 into the liver tissue of Luc tumor mass and injection of HCCLM3-Luc cell suspension through the tail vein, the nude mouse model of human liver cancer was constructed in situ; the nude mouse model of human liver cancer was compared between the mouse model and the optimized model. feasibility?

　　The key elements of the result: The number of HCCLM3-Luc cells is positively correlated (correlation coefficient R2 = 0.9989, linear equation: Y = 1155.8X + 1×106), and the average ROI value is about 140 photons/sec per cell. Is the in situ tumor formation rate [(82.5±7.2)%] of the liver lobe in situ vaccination group significantly higher than that of the tail vein injection group [(34.1±13.2)%]?

　　Conclusion: This project is a nude mouse model of cell line carcinoma in situ. Can a small animal imager for constructing bioluminescent human liver cancer be used to monitor tumor growth in real time?