动物造模,心房颤动模型 z-bio 2021-01-15 356

　　Purpose: Innovatively use microelectrode wires in the traditional traditional high-frequency atrial pacing modeling technology, advanced catheter manipulation system and implantable ECG monitor to track the development of atrial fibrillation and establish atrial fibrillation model.

　　Method: Select 12 adult beagle dogs and divide them into blank control group (6) and atrial fibrosis model group (AF group, 6) randomly? AF Group uses the implant diameter of the SelectSecure system 4.1Fr bipolar solid thin wire 3830, only actively anchors the electrode and connects a special atrial fibrillation model pacemaker to establish atrial high frequency pacing system, in AOO pacing mode Use rapid atrial pacing to establish a postoperative atrial fibrosis model, and use the implantable ECG monitor RevealLINQ tracker to track the development of atrial fibrosis in real time? Using a cardiac color ultrasound imager with a probe transmission frequency of 2.5 MHZ, the experimental dogs were examined by echocardiography, and the left side was measured through the four top cavities. Right atrium area in end systole? Use optical and electron microscopes to observe left atrium tissue?

　　Result: Among the 12 experimental dogs, 2 of the AF group died during the modeling process, and the remaining 10 experimental dogs completed the experiment? The time to successfully establish atrial fibrillation model was (10.63±2.13) weeks. If the atrial fibrosis model is successfully established, then in the AOO mode, the pacemaker atrial stimulation frequency of the high-frequency atrial fibrillation model is (588.75±11.26) times/min? The implanted ECG monitor can accurately and effectively track and record the atrial fibrillation load in the AF group. After successfully creating the atrial fibrosis model, the area of the left atrium was significantly larger than the pre-modeling ((8.20±0.83) cm2 and (3.80±0.08) cm2, P\u003c0.05]; the right atrium. The area of the left atrium in the AF group was larger than Control group [(2.75±0.96) cm2 vs. (4.52±0.44) cm2, P\u003c0.05]; 3.72±0.15) vs. cm2 (8.20±0.83) cm2, P\u003c0.05]; right atrium area Increase [(2.78±0.18) and cm2(4.52) ±0.44) cm2, P\u003c0.05] than the control group? Morphological observations of left atrial tissue sections of atrial fibrillation model dogs and blank control experimental dogs showed that the atrial structure of the atrial fibrillation experimental dogs had been modified.

　　 Conclusion: Can a stable atrial fibrosis model be successfully established using high-frequency atrial pacing? Use the SelectSecure system to implant 3830 bipolar solid atrial pacing electrodes. Allows you to accurately implant electrodes and improve the success rate of electrode implantation in experimental dogs; use the implantable ECG monitor RevealLINQ to monitor atrial fibrosis in real time.