动物造模,人类脑胶质瘤小鼠原位模型 z-bio 2021-01-15 377

　　Objective: How to evaluate the anti-brain tumor effect and safety of the Chinese medicine compound Jinlong capsule extract and its crude polysaccharide.

　　Method: To establish an intracranial orthotopic mouse model to transplant tumor mass into nude mice using surgical orthotopic transplantation technology? Do you want to use the FluorVivoModel100 fluorescence imager to observe brain tumors in all mice through noninvasive real-time imaging? Pass the blank control group (no tumor, no solvent as culture medium)? Negative control group (vehicle) positive control group (temozolomide 32 mg/kg), low-dose JLC extract (750 mg/kg), medium-dose (1200 mg/kg), high-dose group (1500 mg/kg) and crude Polysaccharide (100 mg/kg), compared the tumor inhibition rate of two groups of crude protein (100 mg/kg), and evaluated the effect of forced feeding of Ginron capsule extract on tumor growth in a mouse sympathetic model.

　　Results: 25 days after administration, the tumor inhibition rate of JLC medium and low dose group was 34.63%, which was not significantly different from the negative control group. The tumor inhibition rate of the crude polysaccharide group and crude protein group was 22.26, and the tumor weight was (0.178±0.048) )G±(0.144±0.047). ) G, compared with the negative control group, the positive control group and the high-dose group, there is no significant difference, the tumor inhibition rate is 99.57% -61.90%, and the tumor weight is (0.001±0.001) g? It is (0.088±0.045)g. Is the difference important (P\u003c0.01, P\u003c0.05)?

　　Conclusion: Do the results of this article indicate that JLC crude polysaccharide and crude protein have a specific inhibitory effect on glioma? Tumor burden and drug treatment will affect the normal growth of the weight of mice; there is no obvious acute toxicity at the current dose (P\→ 0.05)?